Journal: Immunity

Article Title: T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

doi: 10.1016/j.immuni.2022.12.010

Figure Lengend Snippet: CD226 signaling is indispensable for IFNγ production from NK cells (A) Intravenous mouse model of 4T1 control or Pvr + metastasis in Il22 −/− mice. Animals received injections of anti-CD226 blocking antibody (420.1, 200 μg per mouse) or control i.p. on days 0, 3, and 14. (B) Numbers of macroscopic lung metastases and colonies in metastasis assay. (C) IFNγ-producing NK cells in the lungs evaluated by flow cytometry. Data presented as means ± SEM; p values <0.05 were considered significant by the Mann-Whitney U test. (D) Representative dot plots depict CD226 low, medium, and high NK cells. Frequency of IFNγ+ cells among NK cell populations (n = 5–6). Numbers represent the frequency of the parent gate. Data presented as means ± SEM; p values <0.05 were considered significant by the two-way ANOVA. (E) Intravenous mouse model of 4T1 control or Pvr + lung metastasis in Il22 −/− mice. Animals received injections of anti-TIGIT agonist antibody (1G9, 250 μg per mouse), anti-CD96 blocking antibody (3.3, 250 μg per mouse), or control i.p. on days 0, 3, and 14. (F) Numbers of macroscopic lung metastases. (G) IFNγ-producing NK and CD8 + T cells in the lungs were evaluated by flow cytometry. Numbers represent the frequency of the parent gate. Data presented as means ± SEM; p values <0.05 were considered significant by unpaired t- test (n = 5–6).

Article Snippet: InVivoMAb anti-mouse CD96 (3.3) , BioXCell , Cat# BE0337; RRID: AB_2894757.

Techniques: Control, Blocking Assay, Flow Cytometry, MANN-WHITNEY

Journal: Immunity

Article Title: T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

doi: 10.1016/j.immuni.2022.12.010

Figure Lengend Snippet:

Article Snippet: InVivoMAb anti-mouse CD96 (3.3) , BioXCell , Cat# BE0337; RRID: AB_2894757.

Techniques: Purification, Control, Virus, Recombinant, Red Blood Cell Lysis, Transfection, Flow Cytometry, DNA Extraction, Gel Extraction, Plasmid Preparation, cDNA Synthesis, Cell Differentiation, Staining, Cell Isolation, Conjugation Assay, Sequencing, CRISPR, Retroviral, Software